Protein Expression and Purification Service

In stock

Guaranteed 1 mg of purified soluble protein for only $500 !

Introduction

AprexBio is pleased to launch the protein expression and purification service. AprexBio has the necessary know-how to deliver the best possible results to our valued customers. We have the capacity to take your project directly from gene synthesis to protein expression and purification.

At AprexBio, we are proud of our ability to optimize protein expression, maximize product yield, and ensure purity to serve every aspect of your research needs.

Protein Expression Systems at AprexBio

1. E. coli Expression System

Escherichia coli (E. coli) is one of the most widely used hosts for the production of heterologous proteins.

The advantages about this system are:
(1) low cost
(2) fast turnaround time
(3) well established purification procedures

2. Drosophila S2 Cell Expression System (DES)

The DES is widely used for the production of precisely matured, folded and processed recombinant proteins. In contrast to E. coli, insect cells are able to incorporate post-translational modifications. This unique tool usually yields high amounts of the target protein, making it highly cost effective in comparison to other eukaryotic expression systems.

3. Mammalian Expression System Mammalian cell lines (CHO, HEK 293, etc.)
The Mammalian Expression System is widely used for the production of recombinant glycoproteins (e.g. vaccines and enzymes) as well as hormones and immunobiologicals (e.g. antibodies, interleukins, and lymphokines), as they have the advantage of full posttranslational modifications of the expressed protein.

Services and Price Summary

Service Name

Details

Deliverables

Price

E coli.

Guaranteed 1mg purified soluble protein
(Cat# APXPEC-1MG)

  • Subcloning
  • Expression optimization and verification
  • Expression and purification
  • QC by SDS-PAGE, Western blot*
  • Tag removal, endotoxin removal service available

Guaranteed 1 mg purified soluble protein

From $500
Request Service, 1mg

E coli.

Guaranteed 5 mg purified soluble protein
(Cat# APXPEC-5MG)

  • Subcloning
  • Expression optimization and verification
  • Expression and purification
  • QC by SDS-PAGE, Western blot*
  • Tag removal, endotoxin removal service available

Guaranteed 5 mg purified soluble protein

From $1050
Request Service, 5mg

Drosophila S2 cells

(Cat# APXP-S2)

  • Subcloning
  • Generation of recombinant vector DNA
  • Establish stable S2 clone
  • Expression and purification of target protein
  • QC by SDS-PAGE, Western blot*
  • Tag removal service available

Purified soluble protein

Please Inquire
Request Service

Mammalian cells

(Cat# APXP-MAMM)

  • Transient expression and purification
  • Or stable cell line establishment
  • Expression and purification of target protein
  • QC by SDS-PAGE, Western blot*
  • Tag removal service available

Purified protein

Please inquire
Request Service

* Western blot, the customer provides antibody to target protein or AprexBio purchases the antibody for the customer.

These Packages have the following features:

  • Customer provides the plasmid or the expression strain/cell lines harboring target gene
  • Size of the target protein is usually under 100kDa
  • In some rare cases, in order to facilitate expression, AprexBio Inc. may try different tags and the final protein product may contain such tag
    The final purified protein is tag-containing
  • AprexBio does not guarantee the endotoxin level of purified protein
  • AprexBio guarantees the protein to be soluble, but not the bioactivity or functionality. If target protein expresses as inclusion body, refolding will be done to make it soluble.

If AprexBio fails to deliver the guaranteed amount due to the nature of the protein or other unpredictable circumstances, only a minimum set up fee will apply.